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KMID : 1005220060210020129
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Journal of Embryo Transfer
2006 Volume.21 No. 2 p.129 ~ p.135
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Effect of In Vitro Maturation Time of Porcine Immature Oocytes on the Subsequent Development and Hatching after In Vitro Fertilization
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Kim Jae-Young
Park Yong-Soo
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Abstract
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This study was conducted to investigate the effect of in vitro maturation (IVM) duration of porcine follicular oocytes on maturation rate, polyspermic rate, and subsequent embryo development. The nuclear maturation rates of oocytes matured for 36, 38, 40, 42 and 44 hr were similar between 68.0, 78.0, 79.5, 73.8 and 81.8% respectively. There was no significant difference in the rates of polyspermy after in vitro feritilization (IVF). The cleavage rate in the group of 36 hr was significantly higher in than that of 40, 44 hr (p<0.05) but not to 38 and 42 hr. The development rate to blastocyst stage was significantly higher in the group of 38 hr (23.1%) than that in the group of 44 hr (15.6%) (p<0.05) but not to 36, 40 and 42 hr. These results suggest that the aged oocytes for 44 hr is not required for the production of bias to cysts derived from porcine IVF embryos.
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KEYWORD
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pig, IVM duration, IVF, embryo development
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